The Laboratory of biological electron microscopy and structural biology

(dr. fei sun’s lab)

 
 
  1. 1.Algebra TOMography package.


  1. 2.Scripts for non-crystallographic and multi-crystal averaging.


  1. 3.Multi-references projection matching for single particle reconstruction.


  1. 4.A GPU-driven particle picking program (AutoMatch) based on template matching algorithm.


  1. 5.VAT4M: the package designed for visualization and analysis of 3D density map (in collaboration with Super Computing Center, CAS)


  1. 6.AtomAlign : An automatic program to align marker-free tilt series of electron tomography.


  1. 7.MarkerAuto: An automatic program to align tilt series of electron tomography based on fiducial markers.


  1. 8.FIRT: Filtered Iterative Reconstruction Technique.


  1. 9.ICON: Iterative Compressed-sensing Optimized Non-uniform fast Fourier transform reconstruction.

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Software and packages for structural biology study

CryoEM experimental data set for testing and education

  1. 1.CryoEM particles of group II chaperonin rATcpn-beta (C9 symmetry)


     Raw particles (CTF corrected) : start.hed, start.img (IMAGIC format)

     Final reconstruction volume: final.mrc


     see Structure, 18(10): 1270-1279.


  1. 2.CryoEM particle of E.coli ribosome (asymmetric particle)


     Raw particles (CTF corrected) : start.hed, start.img (IMAGIC format)

     Final reconstruction volume: final.mrc


     see ACTA BIOPHYSICA SINICA, 26(7): 209-215.


  1. 3. Reconstructed cryoEM maps of Par3-NTD helical filament for model-fitting validation test


  1. a.Maps from none gold standard method:

  2. 1)Reconstructed map 1 from odd data set: threed.5a_part1.mrc

  3. 1)Reconstructed map 1 from odd data set: threed.5a_part2.mrc

  4. b.Maps from gold standard method (available soon)

  5. c.Published atomic model by MDFF fitting method: MDFF_fitting.pdb


     see Structure, 21(6): 997-1006.